FastQCFastQC Report
Thu 28 Apr 2016
ERR1042250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827887
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82000.9904733375448582No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57890.6992500184203883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49500.5979076854691522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44240.5343724445485918No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC44170.5335269185287363No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG34070.4115295928067478No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA26160.31598515256309134No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21290.25716069946743936No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG16670.2013559821569852No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT16510.19942335125445865No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG16120.19471256342955015No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.17562783326710046No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.16995072999092872No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG13870.16753494136277053No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC13700.16548152102883606No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG13060.15775099741872986No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA11770.14216916076710953No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA11070.13371390056855587No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.13286837454870049No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.1306941647833581No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA9830.11873601107397506No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC8980.10846890940430277No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC8510.10279180612813102No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT8300.1002552280685649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTA451.3227755E-424.6666665
GCACCGT400.001930733323.1256
GTATTAC450.00382491820.5555551
CCTAATA450.00382491820.5555552
GGCGGGT555.141424E-420.18181832
GGTTATC1202.382876E-1020.0416662
GTCCTAA609.2338416E-418.51
AGGTTAT1306.9485395E-1018.51
TATAACG500.0070332618.52
CCGTTCT1258.56744E-917.769
CTATTCT650.001579429617.0769234
ACCTTAC650.001579429617.0769238
GCCGTTC1201.0401345E-716.9583328
GGCCGTT1107.801118E-716.8181827
TACGGAT1107.801118E-716.81818227
TTACGGA1107.801118E-716.81818226
GTTACGG1005.8737933E-616.65000225
ATACCGT1708.54925E-1116.323536
CTTGTTA1151.241091E-616.08695622
AAGACGG1151.241091E-616.0869565
Produced by FastQC (version 0.11.5)