FastQCFastQC Report
Thu 28 Apr 2016
ERR1042249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4145730
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131780.3178692293034037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116350.28065021118114303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT88520.21352089981740247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49580.11959293055746516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC3200.017.92187536
TTAACGG3200.017.92187535
CGTATTA3650.016.2191815
CTACGCT803.3852272E-416.18754
TACGACG3550.016.154935
ACGGACC4650.015.9139798
CTAACGC700.002594023215.8571423
GACGGAC4800.015.8020847
GTATTAG13800.015.6847821
TATACTG8600.014.8430235
TTTAGCG2750.014.79999926
TCTAGCG3200.014.45312528
TACTCGC3750.014.30666720
GGTATCA69400.014.234871
GTAGCAC3150.014.0952393
CTCGCTA2101.364242E-1014.09523913
AAGACGG6100.013.950825
TAGCGGG3900.013.75641128
TACGCAG1753.5734047E-813.7428575
TTAGACT4600.013.6739134
Produced by FastQC (version 0.11.5)