FastQCFastQC Report
Thu 28 Apr 2016
ERR1042248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5731459
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT147310.25702007115465714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT111120.19387733559639875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100260.1749292806596017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50000.024.3461
TCTAGCG3750.017.26666628
TACGTAG4750.016.74736814
TTAACGG5250.016.56190535
CTAGCGG4150.016.04819329
GTATTAG19450.015.7892031
GTATCAA78050.015.6438192
AATCGTC4950.015.32323328
GTACGTA5200.015.29807713
TCTATAC10200.015.2352943
TAACGGC5600.015.19642836
TCGTACG750.00410706214.82
CGTAGCG3650.014.69862915
TAACGCC5950.014.6134464
CTAATAC21150.014.4326253
ATTAGAG9750.014.4205123
GTATAGG19000.014.3131581
TAATACT22150.014.0316034
ACGGACC6800.013.8758
AATACTG15850.013.6561515
Produced by FastQC (version 0.11.5)