FastQCFastQC Report
Thu 28 Apr 2016
ERR1042247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643536
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101940.27978315570369006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69320.19025474154777117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64390.17672392972101825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.025.208131
TAGACCG852.7257423E-517.4117645
GACGGAC3250.016.5076927
GTATCAA46400.016.4267252
TAACGGC2250.015.62222136
TTAACGG2150.015.48837235
TCTATAC6000.015.4166673
TTAGCGA1201.9382096E-615.41666627
ACGGACC4000.015.2625018
TAATACT9850.015.2131974
ATTAGAC3650.014.6986313
TATACTG8050.014.4782615
CGACTTA908.2800904E-414.38888910
GTATAGG7750.014.3225791
TCTAGCG2101.364242E-1014.09523828
CTAGCGT800.006302079713.8750014
TCACGTA2800.013.87525
CTAATAC10400.013.6971143
TAATACG3400.013.60294154
TTAGACT4500.013.5666664
Produced by FastQC (version 0.11.5)