FastQCFastQC Report
Thu 28 Apr 2016
ERR1042246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316550
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64350.27778377328354664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43380.18726122898275452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40720.17577863633420387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.11171785629492131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.021.3159921
TATACTG4700.019.6808515
ACGGACC2850.019.4736848
GTTCGCG500.007036985418.511
TACGTAG1409.458745E-1118.514
GACGGAC2750.018.1636357
AAGACGG3550.017.1971845
TAACGGC1554.0199666E-1016.70967936
TAATACT5700.016.228074
TTAGACT2800.015.8571424
GTATCAA35800.015.6061462
GTAAACG1902.7284841E-1115.57894827
TTAACGG1557.219569E-915.516129535
GTATAGG4700.015.3510651
ATTAGAC2350.014.9574473
TAGACAG4750.014.85
GTATTAG7150.014.7482521
TCGTTTA2301.8189894E-1214.47826130
AGACGGA3600.014.3888886
ATACTGT6700.014.358216
Produced by FastQC (version 0.11.5)