FastQCFastQC Report
Thu 28 Apr 2016
ERR1042245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7007234
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT582690.8315549330877204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT523810.7475274837403746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT347410.49578763888861144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281770.4021130163485335No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77820.11105665944650914No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70810.10105271209724122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2750.018.1636355
ACGGACC15300.017.532688
GACGGAC15900.017.2201257
AAGACGG17550.016.6552725
TTCGGGC11150.016.42600835
CAAGACG18350.015.9291554
GCGCAAG17900.015.91621
CGGACCA16900.015.6538459
AGACGGA18950.015.3271776
GTAAACG12000.015.262527
CGCAAGA18150.015.0854012
CGCTTCG12350.014.82995932
TCTAACG1001.0946273E-414.82
GTATTAG16600.014.265061
ACGCTTC13250.013.96226431
CGAACGA8250.013.90302916
ACGAACG8400.013.87515
GGTATCA276950.013.8741661
TAAACGC13400.013.66791128
TATACTG13850.013.6245495
Produced by FastQC (version 0.11.5)