FastQCFastQC Report
Thu 28 Apr 2016
ERR1042244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13433477
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT676000.50322042461531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT672910.5009202010767577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT439090.3268625092371841No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330110.24573682599076918No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA157260.11706574552515332No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC142980.10643558625961097No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT136730.10178303055865581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC30200.018.58
AAGACGG32050.018.4134165
GACGGAC31600.017.6218387
CAAGACG34250.016.8525544
TCTAGCG17550.016.54985828
CGGACCA34650.015.9105339
CGCAAGA35300.015.8796032
CTAGCGG18100.015.84254229
AGACGGA36650.015.69856
TATACCG4850.015.6391755
GCGCAAG36350.015.5226971
TACGACG23950.014.9081425
TCGTTTA19500.014.23076930
GGTATCA395550.014.0310961
GTATTAG30250.014.0049591
GTAAACG19700.013.99238527
TATACTG24350.013.751545
GACGGTA25800.013.6957378
CGACGGT26600.013.562037
ACACGCT45900.013.4215689
Produced by FastQC (version 0.11.5)