FastQCFastQC Report
Thu 28 Apr 2016
ERR1042243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8612058
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT547930.6362358451371322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT442480.5137912447872506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT334030.38786315651845354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275230.3195867933077088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG17400.016.1609215
TCTAGCG8950.015.2960928
GACGGAC17800.015.278097
CTAGCGG9100.015.04395729
ACGGACC18250.015.0027398
AGACGGA19350.014.4366936
CGGACCA19000.014.1184219
TATACTG14800.013.6255
ATACCGT13050.013.4674346
CAAGACG22050.013.2562364
GTATTAG19050.013.2073491
CGAACGA9700.013.15979416
CGAACGT4650.013.1290324
CGGTCCA11400.012.82017510
GGTATCA278400.012.7918471
CGTCGTA10850.012.78801710
GCGCAAG21650.012.7321011
GCGAAAG20750.012.66024118
CGCAAGA21550.012.619492
TACCGTC11900.012.5924377
Produced by FastQC (version 0.11.5)