FastQCFastQC Report
Thu 28 Apr 2016
ERR1042242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836386
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT225232.6928953856233844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128711.5388827646565102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127681.5265678765546051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110831.3251058721690703No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43630.5216490950350675No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.39001131056713045No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28060.3354910292616089No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA27880.33333891289428563No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC27750.33178460662899667No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA26410.3157632958944793No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.31133950113942604No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG23860.2852749806907337No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA20290.24259133940548983No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18880.22573309452812457No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA15780.18866886820200243No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG14930.17850609646742055No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14340.17145193726341665No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG14090.1684628867532455No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.1639195299777854No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA13080.1563871226921541No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.15160464187588027No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12470.14909383944733653No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA12230.14622435095757222No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11950.14287661438618054No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA11230.13426814891688765No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA10540.12601836950881531No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA10460.12506187334556054No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA10030.11992070646806617No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9700.11597515979464028No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9390.11226873716202806No Hit
GTACATGGGATTTATGGAAATGTAGGTAAAAAAAAAAAAAAAA9160.10951881069267062No Hit
GAGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.10186684138663248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACG400.001930749423.1250021
GTACTAG502.7012487E-422.21
CGTCGTA502.7012487E-422.210
CCGTCGT603.7247242E-521.5833349
ACCGTCG555.141485E-420.1818188
AAAAGTA2800.018.52
GGACGGA500.007033317318.56
TAGTACT701.21869365E-418.54
GCGTTAT752.0664181E-417.2666661
ATACCCA752.0664181E-417.2666666
GACTTTT5150.017.242724
AGGACTT5300.016.7547172
CCTTTAT1005.873937E-616.6500021
AGTACTC3350.016.5671635
GGACTTT5450.016.2935773
TTTAATC1251.656008E-716.2799993
CGACCAT803.3811867E-416.187521
GTCGTAG803.3811867E-416.187511
TACTAGG803.3811867E-416.18752
AAAGTAC3250.015.9384613
Produced by FastQC (version 0.11.5)