FastQCFastQC Report
Thu 28 Apr 2016
ERR1042241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867248
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT662991.3621455080982108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT540121.109703060127612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT327180.6722073746807231No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307950.6326983954793346No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80100.16456938294494136No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA74530.1531255444555116No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73020.15002317531385292No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65890.13537424022774266No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65720.1350249668806685No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT54610.11219892637482208No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA52640.10815146464696272No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48960.10059072395735742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC6000.016.657
TATACCG2700.015.7592595
TTAGCGA957.06718E-515.57894827
TATACTG7000.015.3285715
ACGGACC6750.014.5259268
AAGACGG8300.014.4879525
GTATTAG8700.014.459771
GAACAGT30100.014.3820611
GTACTAG3950.014.0506321
AGACGGA7950.013.7295616
GGTATCA267450.013.5023371
ACGAACG3450.013.40579715
ATACCGA3350.013.2537326
CGGTATA2102.046363E-913.21428726
AACAGTG32350.013.1530142
CGACGGT4100.013.0853657
CCGATAA3850.012.9740269
CGGACCA8150.012.938659
CGCAAGA8800.012.8238642
CCTATAC4200.012.773813
Produced by FastQC (version 0.11.5)