FastQCFastQC Report
Thu 28 Apr 2016
ERR1042240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6681585
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT662350.9913067034244121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT576200.862370231015545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT324880.48623193448859814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224190.3355341584369577No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA77240.11560131316147292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC10450.017.703358
AAGACGG12700.017.6259825
GACGGAC11150.017.0896857
CGGACCA11400.016.5526319
GGTATCA245750.016.0571731
AGACGGA13400.015.3246276
CTAACGC2109.094947E-1214.976193
CGCAAGA13400.014.9104482
CGAACGA5250.014.09523816
TCTAACG2151.9826984E-1013.7674432
GTATTAG16350.013.6911331
TATACTG12150.013.5514415
GCGCAAG15300.013.5424831
TACGACG8550.013.4152055
TAACGCC9250.013.44
GTACTAG5250.013.3904751
TCTATAC9750.013.2820513
GCGAAAG13400.013.11567218
CGAGCCG9850.012.95939115
CAAGACG17850.012.9551814
Produced by FastQC (version 0.11.5)