FastQCFastQC Report
Thu 28 Apr 2016
ERR1042238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590797
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115130.7237252773295398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110890.6970719708422884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54760.34422996774572745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35400.22252996453978727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35500.022.4605621
TATGCCG555.144289E-420.18181837
GACGGAC3200.018.57
CAAGACG3850.018.259744
ACGGACC3250.018.2153858
CGCAAGA3600.017.9861092
CGGACCA3400.017.9558819
CCTTATA1552.1827873E-1117.9032272
GCGCAAG3750.017.2666681
TAGACTA1201.0419171E-716.9583345
AAGACGG3600.016.9583325
TAACGCC1901.8189894E-1216.5526314
GTATCAA48100.016.52
TATACTG2600.016.3653855
TAGGAGT1954.1836756E-1115.1794864
CCGGTCG1853.0559022E-1015.020
CGAACGA1752.239176E-914.816
GCGTTAT1508.106508E-814.7999991
CGCATCG1904.5474735E-1014.60526413
TTATACT2550.014.5098044
Produced by FastQC (version 0.11.5)