FastQCFastQC Report
Thu 28 Apr 2016
ERR1042237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4518890
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT874181.9345016143344935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT702421.5544082728280617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT344240.7617799946447026No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289050.6396482321986151No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA79560.17606093531818656No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT62280.13782145615405553No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT52470.116112585170252No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC51910.11487334278993293No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47390.1048708864345005No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA46370.10261369495606222No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46090.10199407376590269No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45710.10115315929354333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45630.10097612466778347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG10850.022.16595
ACGGACC11800.020.5381368
CGCAAGA11550.020.3419912
GACGGAC12100.020.1818187
CGGACCA12500.019.3889
AGACGGA12750.019.2980386
GCGCAAG12900.019.0736451
TCGAACG2950.018.1864413
GGTATCA280050.017.8823431
CAAGACG14500.017.8620684
TACGACG5800.017.2241385
CGAACGT3150.017.0317464
GTACTAG2500.017.021
ACACGCT15000.016.0333339
GTCGGGA2600.015.6538462
TATACTG6250.015.3925
TTAGCGA1455.35656E-815.31034527
GTACCGT855.3684757E-415.2352936
TAACGAA6850.015.12408713
GACCGTG6300.014.976197
Produced by FastQC (version 0.11.5)