FastQCFastQC Report
Thu 28 Apr 2016
ERR1042236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287981
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75422.6189227761553715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66902.32306992475198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32491.1281994298234952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17650.6128876557828468No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.3875255659227519No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.26737875068146855No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6140.21320851028366455No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.1909848219153347No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.18820686086929345No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.1260499824641209No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3060.10625701001107711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT251.231329E-437.025
GGTCAGT200.001839645937.011
CCGAGTT200.001839645937.013
CTCACGA303.5928402E-430.83333424
ACGATCC303.5928402E-430.83333427
ACGGACC1100.030.2727288
GACGGAC1100.030.2727287
CGGACCA1100.030.2727289
GGTGCAC250.005489588729.63
GGTGACC250.005489588729.68
CGAACGC453.9945535E-628.77777930
CGAAAGC1100.028.59090819
CGAGTTC405.924913E-527.75000214
TACGAAC405.924913E-527.75000228
AACGCAC405.924913E-527.75000232
GTCGCCC358.853191E-426.42857237
GACCGTG358.853191E-426.4285727
AGACCGT358.853191E-426.4285726
ACCGTGA358.853191E-426.4285728
GTAAGAC358.853191E-426.4285723
Produced by FastQC (version 0.11.5)