FastQCFastQC Report
Thu 28 Apr 2016
ERR1042235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7212830
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT705710.9784093067492233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT618290.8572086129854717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT329040.4561870999316496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260660.3613838119018471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232050.018.5358771
GACGGAC13250.018.430197
ACGGACC13600.018.3639728
CGGACCA14400.018.1145849
AAGACGG15450.017.1229765
AGACGGA15550.016.0610946
CGCAAGA17000.015.3441182
CGAACGA7450.015.14765116
TCTATAC9200.015.0815223
TAACGGC3600.014.90277936
TATACTG12550.014.7410355
TTAACGG3550.014.59154935
GTATTAG16250.014.5723081
GCGGGTA5250.014.44761923
GCGCAAG18400.014.2771741
TCGTTTA9850.014.27411130
CGGGTAA5500.014.12727224
TCCGATA8400.014.0952398
CCGATAA8200.013.9878049
GTATCAA309400.013.9138642
Produced by FastQC (version 0.11.5)