FastQCFastQC Report
Thu 28 Apr 2016
ERR1042234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4611275
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT171320.3715241446237754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT165630.35918482415384034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96480.2092262985833636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69840.15145485792974828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56550.023.652521
CGCGTAT759.275704E-619.73333412
AAGACGG8450.017.5147935
GTATCAA79850.016.6349412
ACGGACC7850.016.2611478
GACGGAC7900.016.1582287
CGAACGA2750.016.14545416
GTACCGT1151.2441051E-616.0869566
GTATACG1201.9384806E-615.4166671
TACGACG5000.015.175
GTATAGG6950.014.6402871
TAATACT11900.014.6134454
TACCGTC5500.014.4636357
AGACGGA9900.014.2020216
CGGACCA9150.014.1530069
CTAGTAC2750.014.1272723
CGCAAGA9400.013.9734052
TTAGCGA1203.304598E-513.87500127
GACGGAA7750.013.84516235
GACGGTA5350.013.8317778
Produced by FastQC (version 0.11.5)