FastQCFastQC Report
Thu 28 Apr 2016
ERR1042233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7285770
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT326270.44781814413576054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT307900.42260461145493206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172970.23740798844871577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133350.1830280121387307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109950.022.6643941
AAGACGG14900.017.382555
GACGGAC13800.017.0253627
ACGGACC14000.016.9142868
GTATCAA152550.016.298922
CGAACGA6850.016.2043816
CAAGACG17300.015.2919074
TATACTG17700.015.2598875
CGCAAGA16900.015.2159762
TATACCG2700.015.0740735
TACCGTC10700.015.0420567
CGTCGTA9900.014.76262710
CGGACCA16050.014.7538959
GCGCAAG18150.014.6776861
ATACCGT13200.014.5757576
AGACGGA17550.014.1253576
ACACGCT13950.014.0573489
TACGACG8950.014.0558665
CCGTCGT10450.013.9856459
ACCGTCG10700.013.8317768
Produced by FastQC (version 0.11.5)