FastQCFastQC Report
Thu 28 Apr 2016
ERR1042232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759588
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48440.6377141292384819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46670.614412023360032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20950.2758074113861725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14450.19023470618282542No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT9490.12493614959688673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG358.8666135E-426.4285725
TATACCG358.8666135E-426.4285725
GGTATCA13200.025.7878781
ACACCGT451.3226346E-424.6666686
TAAACGG400.001930587123.12500230
GCTAGAC502.7009335E-422.1999993
GACGGAC1850.021.0000027
GAGTACG450.003824630520.5555571
CGTAGAA450.003824630520.5555572
CGTACAC555.1408855E-420.1818183
GTATCAA17200.019.8982562
ACGGACC2000.019.4258
AGACGGA2050.018.9512186
CGGACCA2050.018.9512189
AAGACGG2150.018.9302335
TACACCG902.1502601E-618.55
GGCGTTA500.0070327418.49999831
CGCAAGA2550.018.1372572
GCGCAAG2350.018.1063841
GCAAGAC3650.017.2328783
Produced by FastQC (version 0.11.5)