FastQCFastQC Report
Thu 28 Apr 2016
ERR1042231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063587
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88710.8340643501659948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71480.6720653787607408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46330.43560141295446453No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT23140.21756565283328963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20140.18935921556017515No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.12147572318954633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC4650.027.4516128
GACGGAC4750.026.4842117
AGACGGA5350.024.8971966
CGGACCA5350.024.5514019
GGTATCA23650.024.1712471
AAGACGG5550.024.05
CAAGACG5600.023.4553594
CGCAAGA6100.022.1393452
GCGAAAG5850.022.13675118
CAGAGCG5800.022.00862114
GCGCAAG6100.021.8360651
GTAATAC1700.021.7647063
CGAAAGC5750.021.55652219
TCGCGTA2150.021.5116279
AGCGAAA5950.021.45378317
GTATCAA27750.020.62
AACCGTT450.003825622620.5555577
GAGCGAA6250.020.12816
GTCGCGT2300.020.1086968
CGCGTAA2350.019.68085110
Produced by FastQC (version 0.11.5)