FastQCFastQC Report
Thu 28 Apr 2016
ERR1042230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222288
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20830.9370726265025553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.66670265601382No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.30995825235730223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5140.23123155545958393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGC200.001838743337.09
TGCCTCG401.5923906E-632.37510
AGCTAGC352.3797114E-531.71428735
GGTATCA3800.030.1842121
TATACAT652.9631337E-928.461545
CTCGCCG405.9189202E-527.75000213
GCTAGCA451.3185259E-424.66666836
GTATCAA5000.023.682
TCGCCGC551.8937219E-523.54545414
TAATACC551.8937219E-523.5454544
AGTACTG551.8937219E-523.5454545
TTAATAC400.001926315723.1250023
CTACAGT802.7008355E-823.1250024
GAGCTAG502.69261E-422.234
AAGACTT502.69261E-422.25
GGCCCCA502.69261E-422.224
AGTGCCT603.7089074E-521.5833328
ACTAACA603.7089074E-521.58333211
ACTATAC705.0714316E-621.1428593
AGACCGC705.0714316E-621.14285917
Produced by FastQC (version 0.11.5)