FastQCFastQC Report
Thu 28 Apr 2016
ERR1042229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7220383
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT180110.24944660137834795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT161320.22342305110407576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105950.14673736836397736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57850.023.5367321
TATACCG3100.017.3064525
GTATCAA79800.017.016292
TATACTG18000.014.6972235
TCTATAC14350.014.4390243
TAGACAG15950.013.1065835
CTAATAC15500.012.8903223
TCAACGC108000.012.6074085
CTACACT16450.012.5957444
CTAGTAC5150.012.5728163
ATCAACG109150.012.5254244
CTATACT20550.012.5133834
TAGACTG11300.012.4424785
CAACGCA110050.012.3893686
TACACTG25850.012.1663455
TACGCAG3350.012.1492535
GTATTAG16200.012.10493851
AACGCAG113400.012.0723117
GTACGTA3700.012.013
TATCAAC114700.011.9677423
Produced by FastQC (version 0.11.5)