FastQCFastQC Report
Thu 28 Apr 2016
ERR1042228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5255631
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138670.26385033500259053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT124280.23647017836678413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69910.13301923213406727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.025.2333741
GTATCAA61000.017.2262292
TATACTG12650.015.6482225
TAACGCC3100.014.3225814
TACCGTC2750.014.1272727
TCGACGT800.00630256813.87500112
CGCGTAA950.001246110113.63157910
TAGACAG11300.013.4247795
TAGACTG8150.013.3926395
GACGGAC4050.013.2469147
TCTATAC9400.013.186173
ACGGACC4500.013.1555568
TACACTG19650.012.8982185
ATCAACG82100.012.7990264
TCAACGC82000.012.7695125
TAATCGG3650.012.67123316
GTGTAAG12500.012.581
ATAGGAC8100.012.56172853
TTACACA13850.012.5559574
TATCAAC85100.012.5217393
Produced by FastQC (version 0.11.5)