FastQCFastQC Report
Thu 28 Apr 2016
ERR1042227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6431330
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT248650.3866229846703559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT244550.3802479424940098No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT132420.20589831341262227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96450.1499689799777029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87700.020.820411
TATACTG13900.017.0359735
CTAGTAC5200.016.0096153
GTATCAA116200.015.7138572
CGCGATA1051.6574982E-414.09523914
TCTATAC10250.014.0780493
GACGGAC5950.013.9915977
GTACTAG5800.013.7155171
TTAGCGA1501.3080571E-613.56666727
CGTATAC2501.0913936E-1113.323
ACGGACC6600.013.1742428
TATACCG3250.013.0923075
CTATACT12500.013.0244
GTGTAAG11850.012.9578061
GTATTAG10150.012.7586211
ATACTGT21250.012.6235296
TACTAGG7700.012.4935062
TAGACAG12350.012.4331985
GTATAAG11950.012.3849371
AACGCAG149400.012.34571557
Produced by FastQC (version 0.11.5)