FastQCFastQC Report
Thu 28 Apr 2016
ERR1042226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4124253
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT202490.49097375936927246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT164200.3981327042739619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86650.21009865301667963No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67350.16330229983466096No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT43480.10542515214270318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC8500.020.676477
ACGGACC8900.019.7471928
AAGACGG10550.018.9383895
AGACGGA10650.017.892026
GTAAACG8800.017.44886427
CGGACCA10650.017.3708929
GGTATCA78950.016.9183031
TAAACGC9200.016.89130428
CAAGACG12550.016.509964
CTAGTAC3150.016.4444453
GCGCAAG11250.015.951111
CGCAAGA10900.015.7844042
TTCGGGC9850.015.40101635
CGCTTCG10250.015.34146332
ACGCTTC10150.015.31034431
TAACGCC5950.015.2352934
GTATTAG12450.015.0080321
CACTCTA10750.014.9720949
CGAAAGC11500.014.819
CGACGGT5800.014.6724157
Produced by FastQC (version 0.11.5)