FastQCFastQC Report
Thu 28 Apr 2016
ERR1042225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7357939
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT262910.3573147317475722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT242210.32918185377726017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT146180.1986697633671603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94910.12898992503199605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74650.024.7823161
GTATCAA107900.017.0254862
TCTATAC12450.015.0080323
GTATTAG16400.014.1006111
TATACTG17750.013.8619715
GACGGAC7900.013.8164567
CGAACGA3900.013.75641116
CGCGTAA1753.57486E-813.74285710
CTAATAC16950.013.5339243
CGCGCTA3750.013.3224
CGACGGT5300.013.2641527
CTAGTAC5550.013.03
TAGACTA8400.012.773815
ATCAACG144350.012.7391754
TATACCG3200.012.7187515
AATCGTC5250.012.68571428
ACGGACC8900.012.6797748
TCAACGC145500.012.6512025
CAACGCA146750.012.5560476
ATTAGAC8550.012.5497073
Produced by FastQC (version 0.11.5)