FastQCFastQC Report
Thu 28 Apr 2016
ERR1042224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5660544
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT317390.5607058261538114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT284660.5028845284128168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT151780.2681367727200778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116320.20549261696402324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109100.018.51
TATACTG11950.016.1004185
CTAGTAC5150.015.8058263
CGAACGA1701.4897523E-915.23529416
GTACTAG4700.014.1702121
TCTATAC11450.014.0567683
GTATCAA145650.013.8194292
ACGGACC4100.013.5365858
TCGCTAA2601.8189894E-1213.51923114
ACTGTGC16150.013.5170288
CTAGTCC11800.013.483053
TGTACTA8100.013.4753085
TAGGTCG3550.013.02816921
CGATCGT1857.4001946E-813.013
GTATTAG15950.012.9905951
CTGTGCG7700.012.7337669
CTACACT12400.012.6814514
TGCGACG2653.274181E-1112.56603722
CGCCGTA5500.012.10909225
ACGCGCG2451.5661499E-912.08163421
Produced by FastQC (version 0.11.5)