FastQCFastQC Report
Thu 28 Apr 2016
ERR1042223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5600359
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT228600.4081881179402963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT205500.3669407621904239No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123940.22130724119650186No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85760.15313304022117152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.026.3459071
CGCGATA1002.8798604E-718.514
GTATCAA77450.018.3686262
TATACTG13050.015.8773965
GTACTAG4050.014.6172841
CGAACGA1803.3396645E-914.38888816
TAGACCG908.2810706E-414.3888885
TACACGG3100.013.7258075
TCTATAC9250.013.43
CTATACT13550.013.1070124
TAACCCG2551.6370905E-1113.0588245
TAATACT15700.012.8439494
TAGACTG7700.012.7337665
ATCAACG111650.012.7254824
ATACTGT19800.012.7070716
TCAACGC112250.012.6739425
CTAGACT6900.012.6014494
ATAAGAC8300.012.4819283
CAACGCA114300.012.4790026
TATCAAC115100.012.4404863
Produced by FastQC (version 0.11.5)