FastQCFastQC Report
Thu 28 Apr 2016
ERR1042222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6636833
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192600.29019865348427476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT170130.2563421439111094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT111490.16798674910156697No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66910.1008161573449264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49350.025.903751
GTATCAA74000.017.22
ACGCGCG2500.015.54000121
TATACTG17200.015.4883725
TCTATAC12950.014.5714283
CTAATCG5700.013.9561426
AATCGTC5700.013.63157828
TATATCG4950.013.4545465
GTATTAG16950.013.3156341
TAGACTG11750.013.2255325
TCAACGC101150.012.7113195
GGACTAG14600.012.6712331
ATCAACG101300.012.6194484
TTAGACT8250.012.5575764
TACACTG22050.012.5011335
CTACACT15250.012.4950824
CAACGCA102700.012.4294056
TATCAAC104300.012.4161073
GCGCTAG1050.002645863212.33333311
GACTAGG13700.012.2883212
Produced by FastQC (version 0.11.5)