FastQCFastQC Report
Thu 28 Apr 2016
ERR1042221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642581
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT134610.5093883593350591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT133960.5069286428684683No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62520.23658688229424188No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53660.20305905476501948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.026.832521
GTATCAA43400.019.0967752
TATATCG1306.9849193E-1018.55
TAACGCT701.2199581E-418.54
CGCGCTA855.3674285E-415.23529324
CTATACG1252.9620824E-614.8000014
TACGCAG1252.9620824E-614.8000015
CGAACGC1001.09421824E-414.79999930
TCTAGAC2006.184564E-1114.7999993
CGTCGTT750.004106283714.79999930
TATACTG5750.014.4782615
ATAACGC1304.448935E-614.2307693
TATACCG1501.3072222E-613.5666665
TATCAAC64300.013.1485233
GTATACT4100.013.0853664
ATCAACG64350.012.9083144
CTATACT5900.012.8559324
TAGACTG4200.012.773815
TCAACGC65200.012.740035
CTAGACT2204.090907E-912.6136364
Produced by FastQC (version 0.11.5)