FastQCFastQC Report
Thu 28 Apr 2016
ERR1042219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4363314
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT175020.4011171325281655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT157340.36059747247161217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94140.21575343878529027No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57360.13145971158619343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46650.026.2132911
CTAATCG3150.018.2063526
GTATCAA67350.017.9918352
GACGGAC3450.016.0869567
GTATTAG10550.014.55450251
TATACTG10450.014.51674655
GTACTAG3850.014.4155841
TAATCGT4250.014.36470627
ACGGACC4300.014.1976748
CGCGCTA2101.364242E-1014.09523824
TCTATAC8150.014.0736193
TATACCG1854.9094524E-914.05
AATCGTC4250.013.92941228
ACGCGCG2051.4279067E-913.53658521
TTTAGAC5350.013.4859823
ATAACGC3050.013.3442623
TATATCG3500.013.2142855
ATCAACG91800.013.1797384
TCAACGC92400.013.0941555
TTAGACT5100.013.0588244
Produced by FastQC (version 0.11.5)