FastQCFastQC Report
Thu 28 Apr 2016
ERR1042218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6141773
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT250310.4075533237714908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT248280.404248089273244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138540.22557004304783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89220.1452674984894427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92100.018.8414761
TATACTG13500.016.5814825
GACGGAC7300.016.219187
ACGGACC8000.015.7249998
TCTATAC10550.015.2559243
ACGCGCG4350.014.45976921
CGAACGA2700.014.38888916
GTATCAA121250.014.2202052
TAGACCG1702.4427209E-814.1470595
ACGACGG4200.014.0952396
CGCGCTA4500.013.977777524
GTATACG2800.013.8751
CTATACT15800.013.8164574
TACGACG4600.013.6739125
CGCAAGA9300.013.5268812
TAGACAG11850.013.2700425
CGGACCA9500.013.24210559
AAGACGG10100.013.1881195
GTATTAG12400.012.9798391
GCGCGCT4850.012.96907223
Produced by FastQC (version 0.11.5)