FastQCFastQC Report
Thu 28 Apr 2016
ERR1042217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7240088
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT187240.25861564113585356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177240.24480365432022372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104850.14481868176187915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71850.019.7487831
ACGGACC10650.018.5868558
GACGGAC10700.018.1542077
TATACCG3250.015.369235
CGGACCA13150.015.33469
GCGAAAG12950.015.14285818
AAGACGG16650.014.8888895
TATACTG17400.014.8850585
GTATCAA97550.014.5838032
GTATTAG17450.014.5243561
CGAAAGC13450.014.44237919
ACGCGCG7100.014.33098621
CGCGCTA7250.014.28965624
AGACGGA14900.014.2785236
TCTATAC12500.014.2083
GAGCGAA13850.014.0252716
GTACTAG5450.013.9174311
TACCGTC7750.013.8451627
GCGCAAG14700.013.8435371
CGCAAGA15300.013.7843132
Produced by FastQC (version 0.11.5)