FastQCFastQC Report
Thu 28 Apr 2016
ERR1042216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7491139
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT237180.3166140689686842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT231090.3084844641115323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT135860.18136093857022276No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77910.10400287593115012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.022.878991
AATCGTC6000.016.95833428
GACGGAC6900.016.3550727
ACGGACC7150.016.30078
GTATCAA104950.016.199622
CTAGTAC5500.015.8090913
TAACGCC3400.015.7794124
ACGACGG3750.015.2933326
TATACTG16600.015.0451815
CTAATCG6400.015.0312526
GTATTAG19100.014.916231
CGCAAGA9350.014.443852
TACGACG4550.013.8241755
TATACCG4950.013.4545465
CTACACT20050.013.00997454
TAATCGT7450.012.91275127
CTAATAC19650.012.8040713
TACACTG26600.012.7969925
TAGACTG13200.012.7537885
GTATAAG14450.012.674741
Produced by FastQC (version 0.11.5)