FastQCFastQC Report
Thu 28 Apr 2016
ERR1042215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913386
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT184260.6324599623942725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT142150.48792024125879646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61700.2117810684886932No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59400.20388647436350693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT555.1456987E-420.1818186
CCGTCGT2100.017.6190479
CGTCGTA2100.016.73809410
CGATACG1251.6601552E-716.27999926
TCTATAC6050.016.2066123
CTCTATA4150.015.6024092
CGCGCTA1802.0190782E-1015.41666724
TAACGCC2055.456968E-1215.3414634
AATCGTC2055.456968E-1215.34146328
CTAATCG2055.456968E-1215.34146326
ACGGACC3150.015.2698418
GGTATCA66900.014.711511
TACCGTC2650.014.66037757
TACTCGC1803.3360266E-914.38888920
TTACTCG2101.364242E-1014.09523819
TATACTG6300.014.0952385
TATACCG1601.7861748E-713.8755
TACCCTA5500.013.790915
ACCGTCG2700.013.7037048
TCGATAC1652.5990084E-713.45454525
Produced by FastQC (version 0.11.5)