FastQCFastQC Report
Thu 28 Apr 2016
ERR1042214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4767408
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT150830.31637736900219154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149560.31371344764282816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74190.15561915405604052No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55180.11574423670052993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.021.624361
TACGACG5300.016.405665
TAACGCC4750.016.3578954
GTATCAA66900.015.8176382
ACGACGG5500.015.8090916
ACGCGCG4150.015.60240921
CGACGGT5850.015.1794877
ACGGACC7100.015.1126768
TATACTG11150.015.0986565
GACGGTA6300.014.682548
CGCGCTA4300.014.62790724
TACCGTC5450.014.5963297
CGTCGTA4500.014.38888910
GACGGAC7450.014.1543627
TCGCCGG5550.014.00000117
CCGTCGT4650.013.9247319
CTAATAC12150.013.85596753
CCGGTCG4950.013.82828120
AACGCCG5550.013.6666685
TAATACC8300.013.5963864
Produced by FastQC (version 0.11.5)