FastQCFastQC Report
Thu 28 Apr 2016
ERR1042213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11490173
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT306890.2670891030100243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT298110.2594477907338732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT180340.1569515097814454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105950.021.3723451
ACGGACC20000.016.92758
GACGGAC19250.016.4337657
TTAACGG9450.015.66137635
AATCGTC12200.015.01229628
TCTATAC24100.014.968883
TAACGGC9700.014.87628836
AAGACGG23900.014.8619255
GTATCAA152000.014.82
AGACGGA23050.014.7678966
CGCAAGA23050.014.6876352
TATACTG21200.014.4858495
CGGACCA23300.014.3712459
ACGCGCG11000.014.12727421
CTAATCG13350.013.99625526
CGCGCTA11250.013.97777824
CGAACGT4650.013.9247314
CTAGTAC6700.013.8059713
GTATTAG44100.013.8015861
TACGACG13050.013.75095755
Produced by FastQC (version 0.11.5)