FastQCFastQC Report
Thu 28 Apr 2016
ERR1042212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5010255
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137440.27431737506374426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT129640.2587493051750859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72960.14562133065083513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50550.021.482691
GACGGAC6600.018.7803027
ACGGACC6650.018.3609038
TCTATAC10500.016.2095243
AAGACGG9600.015.9947925
CTAGTCG1059.355554E-615.85714224
AGACGGA7950.015.5911946
TTAACGG2055.456968E-1215.34146335
GTATCAA70450.015.256922
CTCTATA6550.015.2519072
TATACTG10400.015.1201935
TAACGGC2109.094947E-1214.9761936
CCGTCGA1001.0945283E-414.89
GTACTAG3900.014.7051291
TTAGACT6350.014.5669294
CGCAAGA8600.014.412792
CGGACCA8350.014.4011979
GTATTAG13900.014.3741021
GCGAAAG8250.014.12727418
CTAATAC14250.013.8912293
Produced by FastQC (version 0.11.5)