FastQCFastQC Report
Thu 28 Apr 2016
ERR1042211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6843545
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138290.202073632890556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT126200.18440735028409982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81180.11862273134756914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59100.019.6582071
CGTCGTA5800.018.18103410
TACCGTC6550.018.0763367
CCGTCGT6350.016.314969
ACCGTCG6550.016.0992368
TATACTG14450.015.6193775
ATACCGT8950.015.296096
TACGACG6300.015.2698425
CGGATCG2550.015.23529437
CGACGGT6250.015.0967
TCTAGCG4050.015.07407428
GACGGAC9700.015.067017
ACGGACC9650.014.7616598
ACGACGG6400.014.7421886
GACGGTA6750.014.2518518
AATCGTC4550.014.23076928
GTATCAA81300.014.2220172
GTAAACG6150.013.83739927
GTATTAG19150.013.7180151
TTAGACT9950.013.5728654
Produced by FastQC (version 0.11.5)