FastQCFastQC Report
Thu 28 Apr 2016
ERR1042210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7937075
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127780.16099129717181707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT118190.14890876046906448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.017.9535871
ACGGACC15250.016.3770488
GACGGAC15300.015.4771247
GTATTAG21300.015.1995311
TATACTG17600.014.9261365
AAGACGG19150.014.8772845
TAGACCG1508.1203325E-814.85
TAACGCC9450.014.2910054
CGGACCA17700.014.2146899
CTTACAC14200.014.2007053
ATTGACG12300.014.13821232
CGCAAGA18250.013.88767052
AGACGGA18150.013.65840156
TCGTTTA9250.013.630
TAAGACT14150.013.5971734
TTGACGG12700.013.54724433
TCTAGAC8750.013.3200013
TAATACT18750.013.2213334
TCTATAC10500.013.2142873
CGAACGT3650.013.1780824
Produced by FastQC (version 0.11.5)