FastQCFastQC Report
Thu 28 Apr 2016
ERR1042209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7113068
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102010.1434120972834788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99440.13979902905469202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47500.018.967371
GACGGAC10950.017.5707787
ACGGACC12100.016.2066128
TAACGCC7700.015.8571424
AAGACGG13950.015.7813635
TCGTTTA7150.015.52447630
AGACGGA14200.014.9823956
TATACTG14850.014.9494945
ATAACGC9700.014.8762883
TAGACTG10850.014.6635945
TCTAGCG4400.014.29545428
CGGACCA13900.014.2410079
TAGAGTG10450.014.162685
AACGCCG9100.013.6208785
CGCGCTA7200.013.61805524
GTATCAA66450.013.3912722
GTATTAG19500.013.2820511
TATATCG4900.013.2142855
TAATACC12350.013.1821864
TATACCG3800.013.1447375
Produced by FastQC (version 0.11.5)