FastQCFastQC Report
Thu 28 Apr 2016
ERR1042208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6336031
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT144020.22730318080830098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138910.21923819501514433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79320.12518878143115147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66000.017.715151
GTAAACG5550.016.66666627
CGTCGTA5900.016.61864510
TCGTTTA6850.015.93430630
TATACTG14900.015.5201345
ACGGACC12900.015.3449618
TACCGTC7500.015.2933347
TCTAGCG4850.015.25773228
GTATTAG14150.015.1660781
CCGTCGT6600.015.1363649
GACGGAC12850.014.9727637
CGATCGT750.00410712514.813
TAGACAG11550.014.4155855
CGCAAGA15150.014.1650172
AAGACGG14800.014.1255
ATACCGT9700.014.1134026
CTAGCGG5350.013.83177629
TAAACGC6650.013.63157928
CGAACGT3000.013.5666674
ACCGTCG7100.013.5492958
Produced by FastQC (version 0.11.5)