FastQCFastQC Report
Thu 28 Apr 2016
ERR1042207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12841157
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT169550.1320363889328664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT148550.11568272235905222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67700.022.1070881
GACGGAC25350.017.5877727
ACGGACC26050.017.3992318
AAGACGG30200.016.1721865
CGAACGA8250.015.69696916
GTATCAA93800.015.6796382
CGCAAGA31000.015.5758062
AGACGGA31150.014.9662926
CGGACCA30950.014.64458759
TATACTG26300.014.6311795
CAAGACG34750.014.5338134
TACCGTC16550.014.5317217
GTAAACG15550.014.51446827
GTATTAG28250.014.1451331
GCGCAAG34550.014.0289431
ACGAACG8850.014.0056515
TCGTTTA14600.013.93835530
GCGAAAG32800.013.70579318
CGTCGTA15300.013.54248310
TAAACGC17100.013.41520528
Produced by FastQC (version 0.11.5)