FastQCFastQC Report
Thu 28 Apr 2016
ERR1042206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3942149
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103230.26186224823059706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT92240.2339840528605083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62710.15907567167045183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.021.4431821
GACGGAC6900.019.840587
TCTAGCG4000.018.49999828
TTAACGG2050.018.0487835
CGCAAGA8500.017.847062
ACGGACC7900.017.3291138
CGACTTA650.001580854517.07692310
AAGACGG9200.016.6902185
TAACGGC2250.016.44444536
TATACTG6200.016.1129025
CGCAATA4900.015.85714236
AGACGGA9100.015.2472526
GTATCAA55650.015.1922732
TCTACGC1601.0988515E-815.031253
CTAGCGG5050.015.01980229
TACACCG2850.014.9298265
ACGACGG4750.014.8000016
GTATTAG10750.014.7999991
CGGACCA9400.014.7606399
GCGCAAG10050.014.7263681
Produced by FastQC (version 0.11.5)