FastQCFastQC Report
Thu 28 Apr 2016
ERR1042205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11056294
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT503180.4551072900196033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT499580.4518512260980035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT290480.26272817998508363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195050.1764153521966764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA192900.018.3561421
GACGGAC15550.016.6559497
ACGGACC16950.015.4985268
TCTAGCG8350.015.28742528
CTAGTAC8600.015.2732553
TATACTG21550.014.9373555
CTAGCGG8600.014.84302329
AAGACGG20800.013.6971155
GTAAACG11100.013.66666727
GTATCAA259700.013.6132082
CGGACCA19050.013.4986889
GTACTAG8500.013.4941181
CGAACGA7600.013.38815816
TACCGTC11250.012.9911127
ACGAACG7700.012.97402715
TACACTG36650.012.9222375
GTATTAG25200.012.773811
TACGACG10900.012.7293585
TAACGCC10800.012.6759264
AGACGGA21000.012.597626
Produced by FastQC (version 0.11.5)