FastQCFastQC Report
Thu 28 Apr 2016
ERR1042204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6664008
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30850.019.7893031
TCTAGCG5000.017.7628
TATACCG2350.015.7446795
CTAGCGG5600.015.52678729
GTATTAG17300.015.1849711
GTACTAG4400.014.7159091
GACGGAC8950.014.4692737
ACGCGCG5900.014.42372921
CGCGCTA6000.014.18333324
TATACTG12450.014.1164665
ATACCGC8200.013.98780427
ACGGACC9450.013.50793658
TCTATAC9900.013.4545443
GTATCAA45050.013.4284132
TACACCG6550.013.274815
TAGACAG12150.013.094655
CGCAATA7000.012.95000136
GTGTAAG17350.012.9020181
TAACGGC2805.456968E-1212.55357236
TACCGTC6650.012.5187977
Produced by FastQC (version 0.11.5)