FastQCFastQC Report
Thu 28 Apr 2016
ERR1042202.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042202.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5761181
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT398270.6912992318762421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT332870.5777808404214344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT185060.3212188612022431No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114540.19881340301580527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG5850.017.7094021
ACGGACC9450.017.6190478
CTAGTAC6150.016.8455283
TGCGACG4800.016.57291822
GACGGAC10450.015.9330147
TATACTG10850.015.6866365
CGGACCA11450.015.3493449
TCGTTTA6150.015.0406530
TAACGCC8400.014.7559524
TCTAGCG4850.014.49484528
CTAGCGG5000.014.42999929
TAGGTCG5100.014.147058521
TAGTACT8800.014.0852274
TCTACAC9400.013.9734033
CGTCGTA5700.013.9561410
GGTATCA161800.013.8807171
TCTAGAC6700.013.805973
ATGCGAC5900.013.7966121
GTATACG6500.013.6615381
GTGTAAG9900.013.6414151
Produced by FastQC (version 0.11.5)