FastQCFastQC Report
Thu 28 Apr 2016
ERR1042200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6293718
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135060.21459493418675576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT135030.21454726760874893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72030.1144474537944026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA5150.019.3980630
ACGGACC9300.018.58
GACGGAC9550.018.2094237
TAACGCC6800.016.5955894
TATACCG2800.016.5178595
TATACTG13800.016.3550725
CGCAAGA11100.016.02
CGTTTAT7150.015.00699331
CGGACCA12050.014.7385899
AAGACGG13100.014.6870235
AGACGGA12150.014.6172846
ATAACGC8450.014.4497053
TTAACGG2600.014.23076935
CGCATCG7300.014.19178113
CTACACT14950.014.1070244
ATGGTCG7250.014.03448236
TATCGGC1854.9112714E-914.015
GGTATCA76000.013.9723681
GCGAAAG12350.013.78137718
AACGCCG8450.013.355035
Produced by FastQC (version 0.11.5)