FastQCFastQC Report
Thu 28 Apr 2016
ERR1042199.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042199.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8553698
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211980.247822637647483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT197060.23037988949340973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116250.13590613089215917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG14200.015.5035225
ACGGACC11100.015.3333338
TCGTTTA8850.015.25988730
TAACGCC10700.015.0420564
GGTATCA102350.014.6951641
AAGACGG16100.014.1335415
TATACCG4750.013.6315785
GACGGAC12650.013.6007917
GTACTAG7000.013.4785711
ATAACGC12150.013.3991773
TCTATAC13100.013.1335883
CTAGTAC6500.013.0923073
GTATAAG14000.013.0821431
GTATTAG20250.012.9728391
TTAGACT9550.012.5916224
CGCATCG10750.012.56279113
CGCAAGA15250.012.4950822
AATAACG11750.012.4382982
GTATACT10300.012.3932054
ATACCGT12500.012.2846
Produced by FastQC (version 0.11.5)