FastQCFastQC Report
Thu 28 Apr 2016
ERR1042198.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042198.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929411
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53400.27676840237772043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47240.24484156045549654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23480.12169517018406135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.025.1410261
TATACCG705.1048864E-621.1428575
ATAACGC1850.020.03
TAACGCT953.608955E-617.5263164
GTATCAA22550.017.47452
TATACTG4450.016.6292155
TAACGCC1251.6593003E-716.284
TAACGCA803.384053E-416.18754
GTAATAC2750.016.1454543
CGAACGT700.00259334915.8571424
TTAACGG1059.349595E-615.85714235
TCGAACG700.00259334915.8571423
TACGCTG855.3664943E-415.2352945
TCGTTTA1353.9759107E-715.07407530
GTACGTA1353.9759107E-715.07407513
TAACCCG750.004105749514.828
CTAGTAC1001.09397464E-414.83
GCGGGTA750.004105749514.823
AATAACG1508.1094186E-814.82
CGGATCG1152.2112716E-514.4782637
Produced by FastQC (version 0.11.5)