FastQCFastQC Report
Thu 28 Apr 2016
ERR1042197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9487374
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT238990.2519032136816784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT224640.23677784811687616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT153030.16129858483496065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98000.018.028061
GACGGAC10950.016.7260287
ACGGACC12150.015.8353918
TATACCG4550.014.6373635
TACGACG7800.014.230775
TATACTG19250.013.7428565
TCTAGCG6100.013.64754128
CGCGATA950.00124623913.63157914
CGAACGT4250.013.4941184
CGTATTA4700.013.38297915
TCTATAC16950.013.20649053
TAGACAG17150.013.160355
GTATTAG24350.013.0677611
TAGACTG13750.013.050915
GTATCAA134500.013.0118952
TAACGCC8250.013.0060614
CGACGGT8300.012.9277117
CGCAAGA16250.012.8646152
AAGACGG17600.012.718755
GTAGGAC14700.012.7108843
Produced by FastQC (version 0.11.5)